Identification of Stem Cells During Prepubertal Spermatogenesis via Monitoring of Nucleostemin Promoter Activity
نویسندگان
چکیده
منابع مشابه
Identification of stem cells during prepubertal spermatogenesis via monitoring of nucleostemin promoter activity.
The nucleostemin (NS) gene encodes a nucleolar protein found at high levels in several types of stem cells and tumor cell lines. The function of NS is unclear but it may play a critical role in S-phase entry by stem/progenitor cells. Here we characterize NS expression in murine male germ cells. Although NS protein was highly expressed in the nucleoli of all primordial germ cells, only a limited...
متن کاملIdentification of tumor-initiating cells in a highly aggressive brain tumor using promoter activity of nucleostemin.
Controversy remains over whether the cancer stem cell (CSC) theory applies to all tumors. To determine whether cells within a highly aggressive solid tumor are stochastically or hierarchically organized, we combined a reporter system where the nucleostemin (NS) promoter drives GFP expression (termed NS-GFP) with a mouse brain tumor model induced by retroviral Ras expression on a p16(Ink4a)/p19(...
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heteropoly acids (hpa) and their salts have advantages as catalysts which make them both economically and environmentally attractive, strong br?nsted acidity, exhibiting fast reversible multi-electron redox transformations under rather mild conditions, very high solubility in polar solvents, fairly high thermal stability in the solid states, and efficient oxidizing ability, so that they are imp...
15 صفحه اولThe Regenerative Effect of Bone Marrow-Derived Stem Cells in Spermatogenesis of Infertile Hamster
BACKGROUND Infertility is a serious social problem in advanced nations, with male factor in half of all cases of infertility. This study was conducted to determine the regenerative effect of bone marrow-derived stem cells in spermatogenesis of infertile hamster. METHODS Twelve adult male hamsters were equally divided into azoospermic and control groups. Busulfan was intraperitoneally used fo...
متن کاملFunctional Inhibition of Nucleostemin Gene-Acoordinator of Self-Renewal Ability-In Bone Marrow Derived Mesenchymal Stem Cells by Rnai Strategy
Purpose: The aim is to downregulate the expression level of NS as an important factor in sustaining stem cells and certain types of cancer cells self-renewal ability in bone marrow derived mesenchymal stem cells by RNAi strategy and investigate the effects of absence of NS in these cells. Materials and Methods: Double strand NS-specific and control siRNA oligos were designed and transfected in...
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ژورنال
عنوان ژورنال: STEM CELLS
سال: 2008
ISSN: 1066-5099
DOI: 10.1634/stemcells.2008-0506